The San Antonio landmark plays a key role in the film chronicling Pee-wee Herman's search for his stolen bicycle when a devious fortuneteller tells him the bike is located in the Alamo's basement. Pee-wee, played by the late Paul Reubens, learns the Alamo doesn't have a basement, but hasn't stopped tourists from tongue-in-cheek inquiries.

The original screen-used stunt bike from the classic 1985 film “Pee-wee’s Big Adventure” was acquired by the Alamo in San Antonio. The bike played a big role in the movie as Pee-wee Herman, played by Paul Reubens, went to the Alamo in search of his stolen bike.